Open Access Kent State (OAKS)

PURPOSE: To compare the effects of high-intensity interval training (HIIT), sprint interval training (SIT), and moderate-intensity continuous training (MCT) on leukocyte counts and subsets. METHODS: Recreationally active men (n=2; 22±2 yrs) completed a maximal graded exercise test (VO2max) and three exercise trials (HIIT, SIT, and MCT) in a randomized, counterbalanced fashion on a cycle ergometer. HIIT consisted of fifteen 90-second bouts at 85% VO2max interspersed with 90-second active recovery periods. SIT consisted of fifteen 20-second bouts at 130% maximum wattage interspersed with 160-second active recovery periods. MCT was a single continuous bout at 65% VO2max. Each trial lasted 53 minutes, including a 5-minute warm-up and a 3-minute cool-down. Blood was collected before (PRE), immediately post (IP), 30 minutes (30M), 2 hours (2H), 6 hours (6H) and 24 hours (24H) post-exercise. Leukocyte count (WBC), lymphocyte number and ratio (LY# and LY %), monocyte number and ratio (MO# and MO%) and granulocyte number and ratio (GR# and GR%) were analyzed via hematology analyzer. RESULTS: MCT elicited the largest decrease in WBC at 30M compared to the other trials while HIIT led to the largest increase in WBC at IP relative to PRE compared to the other trials. HIIT also elicited the largest decrease in LY# at 2H compared to the other trials. Also, SIT led to a slight decrease in LY# at IP while the other conditions elicited an increase in this subset. CONCLUSION: Preliminary data suggests that MCT may lead to the largest suppression in WBC comparted to HIIT and SIT.

Hematological Effects of a Mechanical aid on Muscular Performance and Recovery following Resistance Exercise

1Tasia A. Doshak, 1Brandon M. Gibson, 1Joseph A. Laudato, 1Cody S. Dulaney, 1Emily C. Tagesen, 2Cardyl P. Trionfante, 1Adam R. Jajtner

1Exercise Physiology Program, Kent State University, Kent, OH

2Department of Kinesiology and Health, University of Miami, Oxford, OH

PURPOSE: To examine the leukocyte response to an acute squat protocol while wearing knee wraps versus no knee wraps. METHODS: 7 resistance trained men (22.3±3.6 yrs, 69.1±1.12 in, 77.9±5.4 kgs BW; 1-RM = 153.3±26.8 kgs) completed four experimental visits. Participants were asked to complete 8 sets of 10 repetitions of the squat at 70% of their 1-RM. Blood samples were collected prior to exercise (PRE), as well as immediately (IP), one hour(1H), 24 hours (24H) and 48 hours (48H) after exercise. Blood samples were analyzed via hematology analyzer for leukocyte count (WBC), lymphocyte (LY), monocyte (MO) and granulocyte (GR) number (#) and ratio (%). Data were analyzed using a condition x time repeated measures ANOVA. RESULTS: Significant main effects of time were observed for LY% (pp2=0.1800), LY# (pp2=0.923), MO# (pp2=0.652), GR% (pp2=0.793) and GR# (pp < 0.05), while GR% was reduced at IP compared to PRE, 1H and 24H (pp < 0.05) compared to PRE, 24H, and 48H. GR% and GR# were also both greater at 24H versus 48H values (pp=0.013). CONCLUSIONS: Resistance exercise elicits an immediate increase in circulating leukocytes, primarily through increases in LY%.