Abundance of virus-like particles (VLP) was determined in Lake Erie subsurface water.The relationship between VLP and the bacterial and phytoplankton communities were investigated. Viral and bacterial numbers were determined using nucleic acid stains and epifluorescent microscopy. Phytoplankton abundance was estimated by chlorophylls extraction. Viral abundance averaged 1.05 x 106 VLP/ml and the ratio of viral to bacterial number was less than 1.0 across most sampling sites and dates. Viral abundance was not correlated with either bacterial abundance or chlorophyll a concentration. Viral abundance was found to be most similar to other Great Lakes and marine systems and dissimilar to other freshwater systems.