α-Synemin contains a unique 312 amino acid insert near the end of its C-terminal tail. Therefore we set out to determine if the insert is a site of protein–protein interaction that regulates the sub-cellular localization of this large isoform of synemin. Yeast-two hybrid analysis indicated that this region is a binding site for the M10 region of titin. This was confirmed with GST pull-down assays. Co-immunoprecipitation of endogenous proteins indicated close association of the two proteins in vivo and immunostaining of cardiomyocytes demonstrated co-localization of the proteins at the M-band of the sarcomere.
Prudner, Bethany C; Roy, Pritam Sinha; Damron, Derek S.; Russell, Mary (2014). α-Synemin Localizes to the M-band of the Sarcomere Through Interaction with the M10 Region of Titin. Elsevier Science 588(24) 4625-4630. doi: 10.1016/J.FEBSLET.2014.11.001. Retrieved from https://oaks.kent.edu/bscipubs/134