Cannabinoid Mediated Inhibition of Ovarian Cancer Cell Proliferation is Mediated via Oxidative stress
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  6. Cannabinoid Mediated Inhibition of Ovarian Cancer Cell Proliferation is Mediated via Oxidative stress
Author(s)
Abstract

I hypothesized that the cytotoxic effects of cannabinoids will be mediated via cannabinoid (CB) receptors and cannabinoids will increase the cellular reactive oxygen species (ROS). SKOV3 cell viability was determined in the presence of WST-1 reagent. Mitochondrial morphology and membrane potential (Δψm) were observed with Mitotracker Green FM and tetramethylrhodamine methyl ester (TMRM). ROS were visualized with 5-(and 6)-chloromethyl-2’,7’-dichlorohydrofluorescein diacetate (CM-H2DCFDA) and dihydroethidium (DHE). The plasma membrane of the cells became permeable to Nuclear Green Dead dye. ROS was also increased in the cannabinoid-treated cells but the cytotoxic effects were not reverse by either CB1 (Rimonabant) or CB2 (AM630) receptor antagonist. Although the antioxidant α-tocopherol (vitamin E) was able to prevent the induction of cytotoxicity, water-soluble Trolox and ascorbic acid were unable to do so.
Format
Conference Proceeding
Publication Date
2018-04-27
Permalink https://oaks.kent.edu/starkstudentconference/2018/Presentations/10
Crawford, B. (2018). Cannabinoid Mediated Inhibition of Ovarian Cancer Cell Proliferation is Mediated via Oxidative stress. https://oaks.kent.edu/node/4956
Crawford, Bert. 2018. “Cannabinoid Mediated Inhibition of Ovarian Cancer Cell Proliferation Is Mediated via Oxidative Stress”. https://oaks.kent.edu/node/4956.
Crawford, B. Cannabinoid Mediated Inhibition of Ovarian Cancer Cell Proliferation Is Mediated via Oxidative Stress. 27 Apr. 2018, https://oaks.kent.edu/node/4956.