Browse the Biology/Ecology Collections
Rhododendron Roots
03/11/2015The Rhododendron genus can be found in many different habitats around the world but scarce in desserts, and dry forests as well as tundra regions. . Rhododendrons are studied from the xylem to the leaves, but we believe the secret is in the roots. Kong et al., found two different dimensions of root trait diameter across 96 subtropical woody species: a diameter related dimension that may integrate root construction, and possibly maintenance and persistence, with a branching density dimension that may express difference in root plastic responses to environment. We would like to address the question of what really goes on in the roots and if the anatomy and morphology of the roots are connected to the temperature tolerance of different rhododendron species. We also believe that Rhododendron with similar cold tolerances have similar fungi on their roots. The study site was the Helen S. Layer Rhododendron garden at Holden Arboretum. We studied the morphology, anatomy of six species of Rhododendron from three sections: Ponticum section (Maximum, Degronianum), Pentanthera section (Austrinum, Molle), and Tsutsuti (Yedoense, Indicum).
Recombination rate and nucleotide diversity in recombination hot-spots in HIV-1 Pol gene from low-income and high-income countries
03/11/2015Identification of recombination hotspots in HIV-1 genome is animportant tool that can be used to predict evolution of the virus and to inform the design of an antiviral drug that avoids targeting the recombination hotspots (e.g. Gao, 2009). Depending on the different selective pressures acting on different genomic regions, recombination can facilitate the accumulation of mutations and accelerate the emergence of resistance to therapies, dissociate favorable combinations of mutations or contribute to emergence of multi-drug resistance bycombining multiple drug resistance mutations in a single recombinant product. The latter is mostly a problem in low- to middle-income countries as multiple subtypes tend to co-circulate in these countries (e.g. Rajaram et al, 2007). For example, under conditions of fast spreading of the virus, such as in standing social intravenous drug user networks, the chances of super-infection, and thus recombination, are greater than that of other transmission modes, suggesting that such epidemics may have a higher rate of virus recombination (Berry et al,2009). We hypothesize that differences in socioeconomic status between countries likely to influence difference in recombination rate of HIV-1 genes that are under strong purifying selection pressure (e.g. Pol gene), which in turn can influence the levels of nucleotide diversity of the virus. In this project we have examined and contrasted the correlationcoefficients between recombination rate and nucleotide diversity of recombination hotspots in Pol gene for low-income and high-income countries. Our study shows that there is a significant difference in recombination events and thus nucleotide diversity in HIV-1 pol gene accounting to socioeconomic status.
Overview of Ebola History and Spread
03/11/2015Survey of evolutionary rate estimates in Ebola virus
Ebola virus first appeared in 1976 in Sudan and Zaire as an illness characterized by fever, severe diarrhea, vomiting and a high fatality rate. Since then there were multiple Ebola outbreaks, some deadlier than others. The ongoing epidemic begun in 2013 in Guinea, and then spread to neighboring countries, leading to over 4900 estimated deaths in the region by late 2014, and continuing to ravage the region. The disease is highly contagious when there is a skin-to-skin contact, with the burial practices exasperating the problem of transmission.
Recent spread of epidemic outside of remote villages underscores the critical need to develop vaccination and/or treatment options to be broadly available in potentially affected areas. However, up to date vaccine development has been challenging , in part because previous outbreaks were relatively small, thus, little was understood about the evolution of the Ebola virus. Currently, more genomes of Ebola are sequenced and available, thus, enabling phylogenetic and molecular evolutionary studies. In order to know whether vaccine or antibody treatments will maintain efficacy, the nature of genetic change in the virus (especially in the targeted regions) must be ascertained. Thus, in this project we survey published estimates of evolutionary rates of various Ebola genes and/or strains to determine (a) the most conserved genes as the most promising vaccine candidates, and (b) whether the rate of evolutionary changes in the virus has increased in recent epidemics compared to previous ones.
Investigating Chemical Stress and Antibiotic Sensitivity of Escherichia coli and Staphylococcus epidermidis
03/11/2015Previous lab work has investigated the effects of thermal stress and antibiotic sensitivity on bacteria used in microbiology teaching labs concentrating on Pseudomonas fluorescens. This investigation, however, focuses on how chemical stress affects the antibiotic sensitivity of two common laboratory strains: Escherichia coli and Staphylococcus epidermidis. Literature searches revealed that potassium acetate is a common additive in sporulation media. Using the Kirby Bauer procedure – a technique used in clinical labs to characterize antibiotic sensitivity of clinical isolates – we studied how the addition of sodium chloride, potassium chloride, sodium acetate and potassium acetate to growth media affects the sensitivity of E. coli and S. epidermidis. After measuring zones of inhibition created by the antibiotic disks, we found that sodium and potassium acetate increased the sensitivity of the two bacteria significantly, while sodium and potassium chloride had negligible effects. Further studies will characterize the relationship between acetate and potential antibiotic sensitivity.
Hypoxic tumor microenvironment alters Vascular Endothelial Growth Factor isoform expression in cancer cells
03/11/2015Vascular endothelial growth factor (VEGF) is necessary for angiogenesis and tumor growth. VEGF splice isoforms have distinct characteristics, with VEGF121 being more diffusible, thus allowing for more distant angiogenic signaling, and VEGF165 and VEGF189 being found within the cell and the extracellular matrix. We hypothesized that VEGF expression in cancer cells would increase under hypoxia and that the VEGF 121 isoform would be enhanced. We examined VEGF mRNA levels and relative isoform ratios in cells grown in hypoxia or treated with CoCl2 to mimic hypoxia. Prostate cancer (PC3, LNCaP) and leukemia (MOLT-4, K562) cells were initially cultured under normoxic conditions with 20% O2 and then placed in a hypoxia chamber with 1% O2 or treated with CoCl2. RNA was extracted from treated and control cells, and quantitative real-time PCR analysis was performed to analyze VEGF expression under the different conditions. Our findings suggest that hypoxia increased both VEGF121 and VEGF165 expression in prostate cancer and leukemia cells. These results of elevated VEGF121 indicate the potential for lethal metastatic tumor growth distant from the primary tumor site. Overall, this work highlights the role of the hypoxic tumor microenvironment in regulating the functionally distinct VEGF isoforms in cancer cells.