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Abstract |
Amber Hydash1, Senay Ustunel1-3, Marianne E. Prévôt 2, Naveen Singhal1, Elda Hegmann1-3 and Robert J. Clements1* 1 Department of Biological Sciences, Kent State University, Kent, OH, USA 2 Liquid Crystal Institute, Kent State University, Kent, OH, USA 3 Chemical Physics Interdisciplinary Program, Kent State University, Kent, OH, USA In vitro studies evaluating neuronal function using cell culture techniques are an invaluable tool. However two-dimensional (2D) studies do not provide accurate details regarding spatial neuronal interactions. We present a platform for long term study of neural networks in vitro using three-dimensional (3D) liquid crystal elastomer (LCE) foams as scaffolds which have proven to be non-cytotoxic to soft tissue cell lines such as, human neuroblastoma cells (SH-SY5Y) and support cell growth for over two months. Treating neurons with retinoic acid (RA) after four weeks of maturation results in an increased neurite length as observed under confocal microscopy. In order to stimulate myelination of neuronal elements in the 3D cultures – glial cells, which support and protect neurons, were seeded on neuroblastoma containing LCE’s. Here we present cell proliferation, viability and cytotoxicity tests assessed for cells grown on the LCE scaffolds. The development of the co-culture will lead to a better representation and understanding of spatial tissue interactions and a suitable method for evaluating therapies for neurodegenerative states. |
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Publication Date |
2018-04-05
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Contributor(s) |
Faculty Mentor
Senay Ustunel Dr. Robert Clements
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Subject | |
Modified Abstract |
In vitro studies evaluating neuronal function using cell culture techniques are an invaluable tool. However two-dimensional (2D) studies do not provide accurate details regarding spatial neuronal interactions. We present a platform for long term study of neural networks in vitro using three-dimensional (3D) liquid crystal elastomer (LCE) foams as scaffolds which have proven to be non-cytotoxic to soft tissue cell lines such as, human neuroblastoma cells (SH-SY5Y) and support cell growth for over two months. Treating neurons with retinoic acid (RA) after four weeks of maturation results in an increased neurite length as observed under confocal microscopy. In order to stimulate myelination of neuronal elements in the 3D cultures – glial cells, which support and protect neurons, were seeded on neuroblastoma containing LCE’s. Here we present cell proliferation, viability and cytotoxicity tests assessed for cells grown on the LCE scaffolds. The development of the co-culture will lead to a better representation and understanding of spatial tissue interactions and a suitable method for evaluating therapies for neurodegenerative states. |
Permalink | https://oaks.kent.edu/ugresearch/2018/2018all/101 |
Cell Proliferation, Viability and Cyto-toxicity Testing on Neuroblastoma Cells with the Use of Liquid Crystal Elastomers
Hydash, A. (2018). Cell Proliferation, Viability and Cyto-toxicity Testing on Neuroblastoma Cells with the Use of Liquid Crystal Elastomers (1–). https://oaks.kent.edu/node/5558
Hydash, Amber. 2018. “Cell Proliferation, Viability and Cyto-Toxicity Testing on Neuroblastoma Cells With the Use of Liquid Crystal Elastomers”. https://oaks.kent.edu/node/5558.
Hydash, Amber. Cell Proliferation, Viability and Cyto-Toxicity Testing on Neuroblastoma Cells With the Use of Liquid Crystal Elastomers. 5 Apr. 2018, https://oaks.kent.edu/node/5558.