Luteinizing hormone (LH) is a heterodimeric glycoprotein gonadotropin that has long been known to play a critical role in reproduction and has recently been linked to changes in cognition and plasticity, especially as LH levels change in the aging process. While researchers have begun to understand some of the effects luteinizing hormone has in the brain, much remains to be explored. Specifically, studies have identified LH protein in several brain regions but it is not clear where it is produced. This study uses hybridization chain reaction in situ hybridization (HCR-FISH) in the mouse brain to identify and localize LH mRNA transcripts. In situ results were combined with immunohistochemistry and rtPCR results to provide a cohesive understanding of where LH is produced versus distributed. Furthermore, analysis of single cell RNA sequencing data identifies what types of cells are responsible for producing LH in regions of interest and suggests possible mechanisms for LH involvement in plasticity. This localization provides a foundation for further experiments to understand the mechanism by which LH affects cognition and plasticity.