Transmission-through-dye (TTD) microscopy makes possible direct measurement of bacterial volume, irrespective of cell shape. The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip.
Journal of Microbiological Methods
Lababidi, Suzanne L.; Pelts, Mariana; Moitra, Moumita; Leff, Laura Gunn; Model, Michael A. (2011). Measurement of Bacterial Volume by Transmission-through-dye Imaging. Journal of Microbiological Methods 87(3) 375-377. doi: 10.1016/j.mimet.2011.08.019. Retrieved from https://oaks.kent.edu/bscipubs/97