Immediately following a disturbing experience, specific contextual cues associated with the experience can elicit the initial unpleasant reaction (e.g., fear). Over time, cues that are similar, but not identical, to the original associated cues can also instigate an aversive response. This phenomenon is known as context generalization, a pathological form of which is frequently a symptom of anxiety disorders such as social anxiety and post-traumatic stress disorder. In order to advance understanding of the pathologies underlying these anxiety disorders, we sought to identify means to re-establish context specificity and reduce fear generalization. Mice and rats were trained in context fear conditioning, and tested at a long interval with or without exposure to the training context. We demonstrated that fear generalization was reduced through a re-exposure to the training context in rats, but not in mice. Our data indicate fear generalization was reduced in both male and female rats. However, fear generalization was only reduced in male mice, but not in females. As females are more susceptible to anxiety disorders, understanding the sex difference in fear generalization is crucial to determining sex-specific treatments for anxiety disorders.