Author(s) | |
---|---|
Abstract |
Molecular typing of Streptococcus pyogenes isolates collected at a Mongolian Hospital (Ullanbaatar, Mongolia) Samantha Mackey1, Dipendra Thapaliya1, Jhalka Kadariya1, Bulgan Davaadash2, and Tara C. Smith1 1 Kent State University, College of Public Health, Department of Biostatistics, Environmental Health Sciences and Epidemiology, Kent, Ohio 2 Bacteriological Reference Laboratory, National Center for Communicable Diseases, Ulaanbaatar, Mongolia Streptococcus pyogenes is a significant cause of morbidity and mortality worldwide, causing an estimated 1.8 million cases and 517,000 deaths each year. S. pyogenes infection disproportionately affects low-income countries where routine surveillance is not available. The objective of this study was to investigate the molecular epidemiology and antibiotic resistance patters of clinically relevant S. pyogenes isolates to better understand the burden in this under-served population. A convenience sample of 41 S. pyogenes isolates collected between 2007 and 2016 by the Bacteriological Reference Laboratory, National Center for Communicable Diseases, Ullanbaatar, Mongolia, and sent to our lab for molecular typing. The emm gene was sequenced and emm type was assigned. We also carried out multi-locus sequence typing (MLST); antibiotic susceptibility testing was carried out via the Vitek-2 system. We observed 18 distinct emm types among the 41 S. pyogenes isolates. stG6792.0 was the most common emm type, accounting for almost one-third of the isolates followed by emm2.0 and emm 82.0. A total of seven sequence types (STs) were detected among 15 tested isolates. The most common ST type was ST55 accounting for one-third of the isolates. Additional MLST analysis is in progress. Most of the isolates were susceptible to all tested drugs. The findings of this study provided some insights regarding the molecular characteristics of S. pyogenes in Mongolia that will be crucial for future surveillance studies. The information about antibiotic susceptibility patterns and molecular types can help to devise better treatment strategies for S. pyogenes infections, and inform vaccine development. Key words: Streptococcus pyogenes, Mongolia, emm typing, multi-locus sequence typing, drug resistant |
Format | |
Publication Date |
2018-04-05
|
Contributor(s) |
Faculty Mentor
Dr. Tara Smith MR. Dipendra Thapaliya
|
Subject | |
Modified Abstract |
Molecular typing of Streptococcus pyogenes isolates collected at a Mongolian Hospital (Ullanbaatar, Mongolia) Samantha Mackey1, Dipendra Thapaliya1, Jhalka Kadariya1, Bulgan Davaadash2, and Tara C. Smith1 1 Kent State University, College of Public Health, Department of Biostatistics, Environmental Health Sciences and Epidemiology, Kent, Ohio 2 Bacteriological Reference Laboratory, National Center for Communicable Diseases, Ulaanbaatar, Mongolia Streptococcus pyogenes is a significant cause of morbidity and mortality worldwide. The objective of this study was to investigate the molecular epidemiology and antibiotic resistance patters of S. pyogenes isolates collected at the Bacteriological Reference Laboratory of Mongolia. The emm gene was sequenced and emm type was assigned. We also carried out multi-locus sequence typing (MLST); antibiotic susceptibility testing was done via the Vitek-2 system. We observed 18 distinct emm types among the 41 S. pyogenes isolates. stG6792.0 was the most common emm type. A total of seven sequence types (STs) were detected among 15 tested isolates. Most of the isolates were susceptible to all tested drugs. The findings of this study provided some insights regarding the molecular characteristics of S. pyogenes in Mongolia that will be crucial for future surveillance studies. |
Comments | |
Permalink | https://oaks.kent.edu/ugresearch/2018/2018all/185 |
Molecular typing of Streptococcus pyogenes isolates collected at a Mongolian Hospital (Ullanbaatar, Mongolia)
Mackey, S. (2018). Molecular typing of Streptococcus pyogenes isolates collected at a Mongolian Hospital (Ullanbaatar, Mongolia) (1–). https://oaks.kent.edu/node/5650
Mackey, Samantha. 2018. “Molecular Typing of Streptococcus Pyogenes Isolates Collected at a Mongolian Hospital (Ullanbaatar, Mongolia)”. https://oaks.kent.edu/node/5650.
Mackey, Samantha. Molecular Typing of Streptococcus Pyogenes Isolates Collected at a Mongolian Hospital (Ullanbaatar, Mongolia). 5 Apr. 2018, https://oaks.kent.edu/node/5650.
The abstract poster and possibly a stand if not provided.