The purpose of this research study was to evaluate autonomic modulation after resistance exercise with and without blood flow restriction (BFR). Autonomic modulation was assessed after low-load bench press with BFR (LL-BFR), traditional high-load bench press (HL), and a control. Assessments included log transformed (ln) total power (lnTP), high-frequency power (lnHF), sympathovagal balance (lnLF:HF ratio), and root mean square of the successive differences (lnRMSSD). Condition by time interactions (p≤0.0150) were demonstrated by a reduction in lnTP, lnHF, and lnRMSSD post LL-BFR and HL compared to Rest and control. A main effect of time (p=0.004) for lnLF:HF ratio was demonstrated by significant augmentation post exercise compared to Rest. These data suggest that LL-BFR and HL significantly reduce vagal modulation up to 30 minutes after resistance exercise.