The world has progressed in many realms, yet bacterial infections are one of the biggest threats to global health contributing to the mortality of numerous humans per year. To the greater extent of the cruelty, it has been shown that antibioticresistant bacteria strains have been causing serious deadly consequences. This study aimed to assess the possibility of using γ-AlOOH nanoparticles incorporated in vitamin K3 composites as an antibacterial agent against Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. Herein, γ-AlOOH NPs were prepared using hydrothermal synthesis following by incorporating with Vitamin K3. After that, the composites were undergone antibacterial activity in vitro determination using Minimum Inhibitory Concentration (MIC) assay and Colony Forming Units (CFU) assay. The results showed that the newly synthesized composites exhibited a robust growth-inhibitory effect in both bacterial strains. In conclusion, the γ-AlOOH nanoparticles incorporated vitamin K3 composites possess the promising ability to develop new antibacterial therapeutics.