Multiple Sclerosis, a neurodegenerative disease is caused by the degradation of oligodendrocytes in the Central Nervous System (CNS) which results in the degradation of the neuronal myelin sheath. This study is about an in vitro model regarding myelination, demyelination, and remyelination of myelin-producing oligodendrocytes. PMA, a cell differentiating agent is administered to study various levels of myelination. Lysolecithin, a cell membrane integrating phospholipid, is used to study demyelination. The delivery of N-acetyl aspartate (NAA) using nanoparticles is used for the study of remyelination. The different levels of myelin production are verified through analysis of myelin-associated mRNA, proteins lipids via qRTPCR, Western Blots, and TLC and GC/MS. The increase in myelin suggests that the remyelination therapy model was successful.