Chronic spinal cord injury (SCI) results in sexual dysfunction in men and rats. Ejaculation is controlled by a spinal reflex generator consisting of lumbar spinothalamic (LSt) cells. These cells convert sensory glutamatergic inputs into a coordinated motor output for ejaculation. Previous work showed SCI significantly reduced glutamate expressing axons. Currently, we tested the time course of the glutamatergic reduction. Male rats received a contusion injury and were later perfused. Confocal analysis showed SCI significantly reduced glutamatergic inputs. It was also determined that similar reductions of glutamate containing axons were noted throughout the entire medial laminae at lumbar spinal levels. These data demonstrate an immediate, long-lasting impact on excitatory glutamatergic inputs to LSt cells and neighboring cells, which may contribute to sexual dysfunction following SCI.