Behavioral dysregulation that may manifest as the use of maladaptive behaviors aimed at regulating or avoiding distress, despite potential negative health consequences, is central to the development and maintenance of common psychological disorders. However, less is known about factors that may influence the engagement of these maladaptive behaviors. Recent research suggests that negative emotion differentiation (NED) may be important. The present investigation was a meta-analysis examining the relationship between NED and maladaptive behaviors ranging from binge drinking and nonsuicidal self-injury to treatment noncompliance, in clinical and nonclinical samples across 17 included studies obtained via electronic literature searches. Despite between-study methodological heterogeneity, our results indicated that NED was negatively associated with the enactment of maladaptive behaviors (r = –.15). Additionally, no significant differences in effect sizes were observed between clinical (n = 7; r = –.15) and nonclinical (n = 10; r = –.16) samples. Critically, the relationship between NED and maladaptive behaviors remained significant even after controlling for negative affect (NA; n = 11; r = –.09). This association also did not depend on levels of NA. Overall, our findings suggest that NED is generally associated with reduced engagement of maladaptive behaviors, regardless of diagnostic status and NA, and have important clinical implications for understanding and treating psychological disorders involving behavioral dysregulation.